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KMID : 0358219940210020165
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Korean Journal of Fertility and Sterility
1994 Volume.21 No. 2 p.165 ~ p.176
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The Effect of the Timing and Dose of Human Chorionic Gonadotropin on Oocyte Recovery, in Vitro Fertilization, and Preimplantation Development in Superovulation of Mouse
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양승희
김향미/오승은/손영수/유한기/우복희
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Abstract
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This study was carried out to investigate the effect of the timing and dose of human chorionic gonadotropin (hCG) on oocyte recovery, in vitro fertilization, and preimplantation development in superovulation of mouse.
F1 hybrid (C57BL×CBA) mice were obtained and superovulation was induced in female mice by sequential intraperitoneal injection of PMSG and hCG.
In the first series of experiments, mice received 5IU of PMSG given intraperitoneally, and 48 hours later were injected 1IU, 5IU, or 10IU of hCG respectively. In the second series of experiments, mice received 5IU of PMSG given intraperitoneally
and
were injected 5IU of hCG 36, 48, or 60 hours later respectively.
1. When the mice received 5IU of PMSG given intraperitoneally and 48 hours later were injected 1IU, 5IU, or 10IU of hCG respectively, there were no differences in the total number of the oocytes obtained from the three experimental groups.
When the cultrues were examined 120hour after termination of insemination the proportion of embryos which had developed to the blastocyst stage was observed to be significantly higher in 10IU hCG group than 5IU hCG group (p<0.05), but there was
no
difference between 10IU hCG group and 1IU hCG group.
2. When the mice received 5IU of PMSG and were injected 5IU of hCG 36, 48, or 60hours later respectively, there were no differences in the total number of oocytes obtained from the three experimental groups.
When cultures were examined 48 hour after the termination of insemination the proportion of unfragmented oocytes which had developed over two-celll stage was observed to be significantly lower in 36hour interval group than 48hour interval and
60hour
interval group (p<0.05).
When the cultures were examined 120hour after termination of insemination the proportion of embryos which had developed to the blastocyst stage was found to be higher in 60 hour interval group than 36 interval or 48 hour interval group (P<0.05),
and the
proportion of hatching blastocyst was found to be higher in 60 hour interval group as well.
In this study, it was concluded that the administration of adequate dose of hCG, and long (60 hour) PMSG-hCG interval were necessary in superovulation of mice (C57BL×CBA) in order to get a large number of oocytes which had an early oocytes which
had an
early embryonic developmental capability when fertilized in vitro, and especially it had better have been avoided to administer a large dose of hCG.
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KEYWORD
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